Areas of expertise:

— Inhibitors and Glues for Protein-Protein Interactions 

— Protein Homeostasis and Molecular Chaperones 

— High Throughput Biophysical Assays 

Selected Publications:

— Pharmacological chaperone for α-crystallin partially restores transparency in cataract models 

— Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation

— Specificity for latent C termini links the E3 ubiquitin ligase CHIP to caspases 

— Mapping interactions with the chaperone network reveals factors that protect against tau aggregation 

— High-throughput screen for inhibitors of protein-protein interactions in a reconstituted heat shock protein 70 (Hsp70) complex

— Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes

— High-throughput discovery of fluoroprobes that recognize amyloid fibril polymorphs

Areas of expertise:

— Chemical proteomics and covalent screening

— Mass spectrometry

— Macrophage phagocytosis 

Selected Publications:

— Improved Electrophile Design for Exquisite Covalent Molecule Selectivity

— Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells

— Phenotypic landscape of an invasive fungal pathogen reveals its unique biology

— RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry 

— CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy

— Macrophages redeploy functional cancer cell surface proteins following phagocytosis 

— P66 is a bacterial mimic of CD47 that binds the anti-phagocytic receptor SIRPα and facilitates macrophage evasion by Borrelia burgdorferi

Areas of Expertise:

- Molecular simulations of proteins and their regulation by lipids and small molecules.
- Mathematical models of complex kinetic systems (stochastic, deterministic, and spatio-temporal)

Selected Publications:

- Remodelling of the translatome controls diet and its impact on tumorigenesis.

- Mitochondrial uncouplers induce proton leak by activating AAC and UCP1.

- Regulation of the mammalian-brain V-ATPase through ultraslow mode-switching.

- Berkeley Madonna Version 10-A simulation package for solving mathematical models.

 

Areas of Expertise:

- Antibody design and selection

- Protease structure, function, and inhibition

Selected Publications:

- Therapeutic Targeting and Structural Characterization of a Sotorasib-Modified KRAS G12C-MHC 1 complex Demonstrates the Anti-tumor Efficacy of Hapten Based Strategies

- Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets

- Identifying protease-activated targets and exploring therapeutic applications

- Multiplex substrate profiling by mass spectrometry for proteases